Pictures

2017

2016

2015

2014

2013

Documents

Protocols from annual meetings
2016
2015
2014
2013
December 2012
July 2012


Charter
Stadgar

Best biobricks

Biobrick name Type Function Year
BBa_K2003000 Protein domain CPF1 2016
BBa_K2003001 RNA CRISPR array for CPF1 2016
BBa_K2003002 Translational unit pBad+RBS+CPF1+Terminator 2016
BBa_K2003010 Coding Inducible Green Fluorescent Protein UnaG+6xHis-tag 2016
BBa_K2003011 Coding Inducible Green Fluorescent Protein UnaG+6xHis-tag+Flexible linker 2016
BBa_K2003012 Coding Inducible Green Fluorescent Protein UnaG+Flexible Linker 2016
BBa_K20030302 Regulatory pBad/araC promoter + medium RBS 2016
BBa_K1688000 Coding Mono-rhamnolipid genes (rhlA and rhlB) including RBS and J23101 promoter 2015
BBa_K1688002 Coding Mono-rhamnolipid gene rhlA including RBS 2015
BBa_K1688003 Coding Mono-rhamnolipid gene rhlB including RBS 2015
BBa_K1688006 Coding A modified laccase (D439A/M510L CueO) with N-His6 tag attached to it via a linker sequence and an export tag (HlyA, BBa_K554002) attached to its C-terminus 2015
BBa_K1688007 Coding A constitutive Anderson promoter assembled with the coding sequence for the modified laccase 2015
BBa_K1688005 Coding The regulatory NahR construct assembled with the coding sequence for dTomato, as well as the coding sequence for the modified laccase 2015
BBa_K1688009 Coding The complete degradation construct with the modified laccase. The NahR construct assembled with the dTomato reporter gene, the modified laccase sequence and the sequence of the dioxygenase. 2015
BBa_K1688010 Coding A constitutive Anderson promoter assembled with the dTomato reporter gene 2015
BBa_K1688012 Coding The NahR construct assembled with the dTomato reporter gene 2015
BBa_K1688013 Coding The naphthalene pathway with the BBa_J23110 promoter and the B0034 RBS 2015
BBa_K1688017 Coding The naphthalene pathway, with the B0034 RBS and both PstI sites mutagenized 2015


To see more biobricks and get more information about the parts, please visit the teams´ wikis. Links can be found on the project page.

Songs and spex

iGEM song book

Do you wanna do assembly?
Melody: Do you want to build a snowman?
Lyrics: Kira Karlsson

Do you wanna do assembly?
Just play the waiting-game all day?
First the restriction has to incubate
So I just sit and wait
Is there no better way?

And then they have to ligate, and I’m still sitting here
watching the hours tick by
*tick tock tick tock tick*

Do you wanna do assembly?
It really has to be assembly
“Go away I’m working!”
Okay fine

Let it grow or Transformation protocol
Melody: Let it go
Lyrics: Kira Karlsson

My cells are competent
the plasmids should be right
The heatshock went just perfect,
co:ncentrations were alright
I spread them on the plate a few hours ago
But something must be wrong, nothing seems to grow

Add DNA and add LB,
Follow proto-colls and you will see,
Conceal don’t sneeze, don’t do it wrong,
It won’t go wrong!

Let it grow, let it grow
I streaked them on 12 hours ago
Oh just grow, f*cking grow!
I just need to know
Did transformation work?
Or must I start again?
I just want to go home!
*This plate is not with chloramphenicol*

Wiki freeze is comming

Melody: Game of thrones intro
Lyrics: Kira Karlsson

It’s time - for the dreaded - wiki-freeze
(wiki, wiki freezing, wiki,wiki, wiki-freezing, wiki….)
The last chance - to make up stuff - for the gold
(wiki, wiki freezing, wiki,wiki, wiki-freezing, wiki….)

the whole night - looking for bugs in the code
(wiki, wiki freezing, wiki,wiki, wiki-freezing, wiki….)
changing - paraphrasing- in the text
(wiki, wiki freezing, wiki,wiki, wiki-freezing, wiki….)

In hours - everything must - be writ down
(wiki, wiki freezing, wiki,wiki, wiki-freezing, wiki….)
Still TIME for one last experiment
(wiki, wiki freezing, wiki,wiki, wiki-freezing, wiki….)

Are there - spellingmisstakes
That - I’ve yet to find
(wiki, wiki freezing, wiki,wiki, wiki-freezing, wiki….)

Must finish my - eastereggs
Before the freeze
(wiki, wiki freezing, wiki,wiki, wiki-freezing) WIKI….

O hemska lab
Melody: O helga natt
Lyrics: Unknown, Nils Anlind and Kira Karlsson

O, hemska labb, o grymma kval imorgon.
Här sitter jag och förstår ingenting.
Hela min hjärna känns centrifugerad
och något ljushuvud slängde fel fraktion
hur ska jag nu kunna extrahera
när etanolen jag har supit upp

Kristallisera, fäll ut och separera
O hemska lab , jag fick bagsug nu igen.
O hemska lab, nu vill jag åka hem

O, hemska lab, o grymma kval imorgon.
Här sitter jag och förstår ingenting.
Hela kultuuuuren är kontamineeeeerad
åh primer dimers är min enda produkt
hur kan jag nu klippa och liggera
när en-zy-mer-na alla har tatt slut

Åhh transformeeras, geeeeener extraheeerras
O hemska lab , hur ska vi vinna guld?
O hemska lab, tar sommarn aldrig slut
Always look on the bright side of lab
Melody: Always look on the bright side of life
Lyrics: Nils Anlind and Martin Friberg

Some things in lab are bad
They can really make you mad
Some protocols just make you swear and curse
When you're chewing on life's gristle
Don't grumble, give a whistle
And this'll help things turn out for the best…

And...always look on the bright side of lab...
(Whistle)
Always look on the light side of lab...
(Whistle)

If lab seems jolly rotten
There's something you've forgotten
And that's to add your template DNA
When you're curisng at the dimers
Doublecheck your primers
Use touchdown protocol
-yea, that's the thing.

And...always look on the bright side of lab...
(Whistle)
Always look on the right side of plates...
(Whistle)

For gels are quite absurd
And bands the final word
You must always face the screening with a bow
Forget about your gene
Don’t think of where it’s been
Sequence - it's your last chance anyhow.

So always look on the bright side of fail...
(Whistle)
Just before you draw your terminal breath...
(Whistle)

Transformations shit, when you look at it
Ligation fail, and enzyme's are a joke,
You'll see its all a show, keep on testing as you go
Just remember that the last test is on you

And...Always look on the bright side of life...
(Whistle)
Always look on the right side of life...
(Whistle)
Always look on the bright side of life...
Always look on the bright side of life...

Vill du labba med mig
Melody: Om sanningen ska fram (Vill du ligga med mig då)
Lyrics: Nils Anlind

Om jag ger dig ett projekt,
inte labbar på det själv.
Om jag ger dig tips i labbet,
men det spelar ingen roll.
För perfektion finns i pipetten,
om du använder den väl,
Om du följer labb receptet,
inte går din egen väg.

Vill du labba med mig då, pipettera med mig då
autoklavera med mig då, ligera med mig då
Vill du labba med mig då, transformera med mig då
inkubera med mig då, extrahera med mig då

Om jag ger dig mina celler,
och sen lite reagens,
Transformerar in konstruktet
kör en screening på dem sen.
Om jag får korrekta banden
på min gel från PCR
Om du skickar in konstrukten,
till vår bio databank

Vill du labba med mig då, pipettera med mig då
autoklavera med mig då, ligera med mig då
Vill du labba med mig då, transformera med mig då
inkubera med mig då, extrahera med mig då!